To clean the system.
Waters hplc system cleaning procedure.
Cleaning mixtures for lc table in the controlling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307.
Residues or contaminations may block filters or capillaries.
Ion exchange to remove any remaining ions.
Remove all solvent filters.
Water flush to clean acid from the system.
10 nh4oh in water this removes peg and other.
Water acetonitrile methanol ipa 1 1 1 1 with 2 5 fa.
Disconnect the column and attach a union and flow directly to waste.
Use solvent inlet filters to protect the system from incoming particles.
2 0 scope this procedure is applicable for of all high performance liquid chromatography installed in quality control laboratory.
Technical assistant 3 2 checking.
To clearly lay down the procedure for cleaning.
Water flush to clear customer s mobile phases from the system.
Label bottles correctly with bottle content and filling date expiry date.
Maintaining your hplc uhplc uplc systems.
Ultrapure water is water that has been purified through a system that targets contami nants detrimental to uplc ms and hplc ms systems.
When working with any lc system setting it up and storing it is very important.
For information on other acquity uplc cleaning methods refer to the recommended cleaning mixtures for lc table in the con trolling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307.
The water flushes are only needed to remove the previous solvent from the system.
Executive manager 4 0 accountability head of the department 5 0 procedure 5 1 for system cleaning.
3 0 responsibility 3 1 doing.
Objective to lay down the procedure for operation of hplc waters alliance 2695 separation module scope to ensure that the instrument performs satisfactory and gives accurate and reproducible data.
To clean the system.
Place a b c and d seal wash and needle wash lines into a clean.
Use only hplc grade solvents and water filtered through 0 2.
Cleaning decontamination columns general guidelines flow cells general.
Controlling contamination in lc ms and hplc ms systems 715001307.
This sop shall be applicable for the hplc system waters alliances 2690 2695 separation modules.
It is necessary to restore the system flow path to a near new level of cleanliness note.
Responsibility quality control officer executive.
No injections are needed with that.
Flush the system using one of the cleaning mixtures as described in the cleaning solution table of the controlling contamination in uplc ms and hplc ms sytems guide 715001307.
